ONCOLOGY / BASIC RESEARCH
MicroRNA 144 inhibits cell migration and invasion
and regulates inflammatory cytokine secretion
through targeting toll like receptor 2 in non-small cell
lung cancer
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1
Department of Laboratory, The Third People’s Hospital of Dongguan, Dongguan,
Guangdong, China
2
Department of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian, China
3
Department of Education and Science, The Third People’s Hospital of Dongguan,
Dongguan, Guangdong, China
Submission date: 2018-02-24
Final revision date: 2018-05-31
Acceptance date: 2018-06-14
Online publication date: 2020-03-10
Publication date: 2021-07-16
Arch Med Sci 2021;17(4):1028-1037
KEYWORDS
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ABSTRACT
Introduction:
MicroRNAs (miRNAs) are endogenous small noncoding RNA molecules involved in modulation of cancer progression. Here, we investigated the possible role of miR-144 in non-small cell lung cancer (NSCLC) development.
Material and methods:
The expression of miR-144 and TLR2 in NSCLC tissue and cell lines was determined by quantitative real-time PCR (qPCR). The TargetScan database was used to predict potential target genes of miR-144. Luciferase assay was used to verify the interaction between TLR2 and miR-144. TLR2 protein expression was measured by western blot. The secretion of interleukin (IL)-1β, IL-6 and IL-8 in A549 cells was detected by an ELISA kit. Cell migration and invasion were evaluated by wound healing assay and transwell assay, respectively.
Results:
Our results showed that miR-144 was downregulated in NSCLC tissue and cell lines when compared with the normal tissues and cell line (p < 0.05). The protein level of TLR2 in NSCLC tissue and cell lines was significantly higher than that in normal lung tissues. Dual luciferase reporter gene assay showed that miR-144 could bind to the 3ʹUTR of TLR2 specifically. Up-regulation of miR-144 significantly decreased the expression of TLR2. Up-regulation of miR-144 or down-regulation of TLR2 could decrease cell migration, invasion and secretion of IL-1β, IL-6 and IL-8 in A549 cells. Moreover, overexpression of TLR2 rescued the inhibitory effects of miR-144 on migration, invasion and inflammatory factor secretion of A549 cells.
Conclusions:
miR-144 could inhibit the migration, invasion and secretion of IL-1β, IL-6 and IL-8 through downregulation of TLR2 expression in A549 cells.