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ONCOLOGY / RESEARCH LETTER
Combretastatin A4 phosphate encapsulated in hyaluronic acid nanoparticles is highly cytotoxic to oral squamous cell carcinoma
1
Department of Orthodontics, The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi Province, China
2
Jiangxi Province Key Laboratory of Oral Biomedicine, Nanchang, Jiangxi Province, China
3
Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang, Jiangxi Province, China
4
Department of Cariology and Endodontics, The Affiliated Stomatological Hospital of Jiujiang University, Jiujiang, Jiangxi Province, China
5
Department of Stomatology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China
6
Department of General Dentistry, The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi Province, China
These authors had equal contribution to this work
Submission date: 2023-12-21
Final revision date: 2024-05-31
Acceptance date: 2024-05-31
Online publication date: 2024-06-30
Arch Med Sci 2024;20(3):1022-1028
KEYWORDS
TOPICS
ABSTRACT
Introduction:
To investigate the toxicity of combretastatin A4 phosphate (CA4P) hyaluronic acid (HA) gel nanoparticles (HA-CA4P-NPs) in OSCC (oral squamous cell carcinoma).
Methods:
Toxicity was investigated using fluorescence microscopy, MTT as-say, flow cytometry, and OSCC xenograft mouse models.
Results:
Compared with CA4P, HA-CA4P-NPs generated nearly 10 times more fluorescence in OSCC cells. Cytotoxicity assays showed that HACA4P-NPs were more toxic to SCC-4 cells but not to HNECs. Remarkable necrosis was induced in SCC-4 cells after exposure to HA-CA4P-NPs, and related proteins were upregulated. Furthermore, HA-CA4P-NPs significantly reduced the tu-mour size.
Conclusions:
HA-CA4P-NPs improved drug release and delivery, and in-creased cytotoxicity to cancer cells.
To investigate the toxicity of combretastatin A4 phosphate (CA4P) hyaluronic acid (HA) gel nanoparticles (HA-CA4P-NPs) in OSCC (oral squamous cell carcinoma).
Methods:
Toxicity was investigated using fluorescence microscopy, MTT as-say, flow cytometry, and OSCC xenograft mouse models.
Results:
Compared with CA4P, HA-CA4P-NPs generated nearly 10 times more fluorescence in OSCC cells. Cytotoxicity assays showed that HACA4P-NPs were more toxic to SCC-4 cells but not to HNECs. Remarkable necrosis was induced in SCC-4 cells after exposure to HA-CA4P-NPs, and related proteins were upregulated. Furthermore, HA-CA4P-NPs significantly reduced the tu-mour size.
Conclusions:
HA-CA4P-NPs improved drug release and delivery, and in-creased cytotoxicity to cancer cells.
REFERENCES (17)
1.
Rosenthal EL, Kulbersh BD, Duncan RD, et al. In vivo detection of head and neck cancer orthotopic xenografts by immunofluorescence. Laryngoscope 2006; 116: 1636-41.
2.
Mohammed ES, El-Beih NM, El-Hussieny EA, et al. Effects of free and nanoparticulate curcumin on chemically induced liver carcinoma in an animal model. Arch Med Sci 2021; 17: 218-27.
3.
Jain RK, Baxter LT. Mechanisms of heterogeneous distribution of monoclonal antibodies and other macromolecules in tumors: significance of elevated interstitial pressure. Cancer Res 1988; 48: 7022-32.
4.
Sun Q, Zhou Z, Qiu N, et al. Rational design of cancer nanomedicine: nanoproperty integration and synchronization. Adv Mater 2017; 29(14). doi: 10.1002/adma.201606628.
5.
Gao M, Deng H, Zhang W. Hyaluronan-based multifunctional nano-carriers for combination cancer therapy. Curr Top Med Chem 2021; 21: 126-39.
6.
Pan W, Qin M, Zhang G, et al. Combination of hydrotropic nicotinamide with nanoparticles for enhancing tacrolimus percutaneous delivery. Int J Nanomed 2016; 11: 4037-50.
7.
You J, Wang L, Yang F, et al. The ultra-performance liquid chromatography tandem mass spectrometry method for detection and quantification of C4NP in rat plasma and its application to pharmacokinetic studies. Curr Oncol 2016; 23: e8-16.
9.
Mirabello G, Lenders JJ, Sommerdijk NA. Bioinspired synthesis of magnetite nanoparticles. Chem Soc Rev 2016; 45: 5085-106.
10.
Ma X, Gong N, Zhong L, et al. Future of nanotherapeutics: targeting the cellular sub-organelles. Biomaterials 2016; 97: 10-21.
11.
Gharat SA, Momin M, Bhavsar C. Oral squamous cell carcinoma: current treatment strategies and nanotechnology-based approaches for prevention and therapy. Crit Rev Ther Drug Carrier Syst 2016; 33: 363-400.
12.
Irani S, Shahmirani Z, Atyabi SM, et al. Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles. Arch Med Sci 2015; 11: 1286-95.
13.
Ramasamy T, Tran TH, Choi JY, et al. Layer-by-layer coated lipid-polymer hybrid nanoparticles designed for use in anticancer drug delivery. Carbohydr Polym 2014; 102: 653-61.
14.
Zhu SW, Wang S, Wu ZZ, et al. Overexpression of CD168 is related to poor prognosis in oral squamous cell carcinoma. Oral Dis 2022; 28: 364-72.
15.
Liu Y, De Keyzer F, Wang Y, et al. The first study on therapeutic efficacies of a vascular disrupting agent CA4P among primary hepatocellular carcinomas with a full spectrum of differentiation and vascularity: correlation of MRI-microangiography-histopathology in rats. Int J Cancer 2018; 143: 1817-28.
16.
Peng P, Nie Z, Sun F, et al. Glucocorticoids induce femoral head necrosis in rats through the ROS/JNK/c-Jun pathway. FEBS Open Bio 2021; 11: 312-21.
17.
Li C, Shen J, Wei X, et al. Targeted delivery of a novel palmitylated D-peptide for antiglioblastoma molecular therapy. J Drug Target 2012; 20: 264-71.
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| eISSN: | 1896-9151 |
| ISSN: | 1734-1922 |
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